Roles of cholesterol and sphingolipids in formation of functional caveolae were assessed using a 44 cholesterol chelator, methyl-beta-cyclodextrin (MβCD) and the de novo sphingolipid synthesis

8 Departments of Physiology and Biophysics, Medicine, Kidney Disease Program, 9 Biochemistry and Molecular Biology, University of Louisville, School of Medicine, Louisville, 10 KY and Department of Pathology, Wayne State University, School of Medicine, Detroit, MI 11 12 13 14 Running title: Sphingolipids in Fg-induced cerebrovascular permeability 15 16 17 * Drs. Muradashvili and Khundmiri are equally contributed first authors. 18 19 20 21 **Correspondence: 22 Dr. David Lominadze 23 University of Louisville 24 Dept. of Physiology & Biophysics 25 School of Medicine, Bldg. A, Room 1115 26 500 South Preston Street 27 Louisville, KY 40202, USA 28 Phone (502) 852-4902, Fax (502) 852-6239 29 [email protected] 30 31 Articles in PresS. Am J Physiol Cell Physiol (May 14, 2014). doi:10.1152/ajpcell.00305.2013